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KMID : 1007520080170061391
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Food Science and Biotechnology
2008 Volume.17 No. 6 p.1391 ~ p.1395
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Characterization of Low Temperature-adapted Leuconostoc citreum HJ-P4 and Its Dextransucrase for the Use of Kimchi Starter
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Kim Chang-Young
Eom Hyun-Ju
Jin Qing
Kim So-Young
Han Nam-Soo
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Abstract
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Leuconostoc citreum HJ-P4 is a strain isolated for kimchi fermentation with its low temperature-adapted growthfeature and its high dextransucrase activity. The detailed characteristics of cell growth and dextransucrase activities wereinvestigated at various environmental conditions such as temperatures, pHs, salts, and raw ingredients. This strain showedalmost 2-fold higher maximal cell concentration (Xmax) than that of the type culture Leuconostoc mesenteroides B-512F at10oC. The Xmax of the strain was maximum at pH 7 and the cell growth was inhibited by salts in a dose-dependent mode up to7%. Addition of pepper (<6%), garlic (<10%), and ginger (<2%) in kimchi gave no inhibition effect on the growth of HJ-P4.Dextransucrase synthesized by this strain retained over 80% of its maximum activity at 10oC showing a comparable cold-adapted feature to its host microbe. This culture can be used as a starter culture in the industrial kimchi production givingdesirable functions and predominance at low temperature.
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KEYWORD
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Leuconostoc citreum, dextransucrase, low temperature, kimchi
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